Perform ECL, ECF or infrared detection as described by the manufacturer, e.g.Wash the membrane in 0.1% TBST twice for 5 min, and twice for 10 min.Incubate the membrane with the secondary antibody for 1 hr at RT again diluted as recommended in 1% BSA / 0.1% TBST.Rinse the blots briefly in 0.1% TBST and then perform two 5 min washes followed by two 10 min washes using the same buffer.Incubate the membrane with the primary antibody for 1.5 hr at RT or overnight at 4☌. Add blocking peptides (at 1 μg/ml) as required and incubate on a rotating platform for 20 min at RT. Cut the membrane into strips if necessary and prepare the primary antibody by diluting in blocking buffer (5% BSA / 0.1% TBST) at a dilution recommended by the Abcam datasheet.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |